2018
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V Vitali, T Yang, P Minzioni Separation efficiency maximization in acoustofluidic systems: Study of the sample launch-position Journal Article RSC Advances, 8 (68), pp. 38955–38964, 2018. Abstract | Links | Tags: Microfluidic chip, Optofluidics @article{Vitali2018,
title = {Separation efficiency maximization in acoustofluidic systems: Study of the sample launch-position},
author = {V Vitali and T Yang and P Minzioni},
doi = {10.1039/c8ra08860h},
year = {2018},
date = {2018-01-01},
journal = {RSC Advances},
volume = {8},
number = {68},
pages = {38955--38964},
abstract = {The development of lab-on-chip microfluidic systems based on acoustic actuation, and in particular on the acoustophoretic force, has recently attracted significant attention from the scientific community thanks, in part, to the possibility of sample sorting on the basis of both geometrical and mechanical properties. It is commonly recognized that sample prefocusing and launch-position optimization have a substantial effect on the performance of these systems but a clear explanation of how these two parameters influence the system efficiency is still missing. In this manuscript we discuss the impact of both the sample launch position and the sample distribution at the input by the theoretical analysis of a simplified system and by numerical simulations of realistic configurations. The results show that the system performance can be greatly improved by selecting the proper microchannel dimensions and sample-launch position, offering relevant guidelines for the design of micro-acoustofluidic lab-on-chip devices.},
keywords = {Microfluidic chip, Optofluidics},
pubstate = {published},
tppubtype = {article}
}
The development of lab-on-chip microfluidic systems based on acoustic actuation, and in particular on the acoustophoretic force, has recently attracted significant attention from the scientific community thanks, in part, to the possibility of sample sorting on the basis of both geometrical and mechanical properties. It is commonly recognized that sample prefocusing and launch-position optimization have a substantial effect on the performance of these systems but a clear explanation of how these two parameters influence the system efficiency is still missing. In this manuscript we discuss the impact of both the sample launch position and the sample distribution at the input by the theoretical analysis of a simplified system and by numerical simulations of realistic configurations. The results show that the system performance can be greatly improved by selecting the proper microchannel dimensions and sample-launch position, offering relevant guidelines for the design of micro-acoustofluidic lab-on-chip devices. |
2016
|
T Yang, F Bragheri, G Nava, I Chiodi, C Mondello, R Osellame, K Berg-Sørensen, I Cristiani, P Minzioni A comprehensive strategy for the analysis of acoustic compressibility and optical deformability on single cells Journal Article Scientific Reports, 6 , 2016. Abstract | Links | Tags: Integrated, Microfluidic chip, Optofluidics @article{Yang2016a,
title = {A comprehensive strategy for the analysis of acoustic compressibility and optical deformability on single cells},
author = {T Yang and F Bragheri and G Nava and I Chiodi and C Mondello and R Osellame and K Berg-Sørensen and I Cristiani and P Minzioni},
doi = {10.1038/srep23946},
year = {2016},
date = {2016-01-01},
journal = {Scientific Reports},
volume = {6},
abstract = {We realized an integrated microfluidic chip that allows measuring both optical deformability and acoustic compressibility on single cells, by optical stretching and acoustophoresis experiments respectively. Additionally, we propose a measurement protocol that allows evaluating the experimental apparatus parameters before performing the cell-characterization experiments, including a non-destructive method to characterize the optical force distribution inside the microchannel. The chip was used to study important cell-mechanics parameters in two human breast cancer cell lines, MCF7 and MDA-MB231. Results indicate that MDA-MB231 has both higher acoustic compressibility and higher optical deformability than MCF7, but statistical analysis shows that optical deformability and acoustic compressibility are not correlated parameters. This result suggests the possibility to use them to analyze the response of different cellular structures. We also demonstrate that it is possible to perform both measurements on a single cell, and that the order of the two experiments does not affect the retrieved values.},
keywords = {Integrated, Microfluidic chip, Optofluidics},
pubstate = {published},
tppubtype = {article}
}
We realized an integrated microfluidic chip that allows measuring both optical deformability and acoustic compressibility on single cells, by optical stretching and acoustophoresis experiments respectively. Additionally, we propose a measurement protocol that allows evaluating the experimental apparatus parameters before performing the cell-characterization experiments, including a non-destructive method to characterize the optical force distribution inside the microchannel. The chip was used to study important cell-mechanics parameters in two human breast cancer cell lines, MCF7 and MDA-MB231. Results indicate that MDA-MB231 has both higher acoustic compressibility and higher optical deformability than MCF7, but statistical analysis shows that optical deformability and acoustic compressibility are not correlated parameters. This result suggests the possibility to use them to analyze the response of different cellular structures. We also demonstrate that it is possible to perform both measurements on a single cell, and that the order of the two experiments does not affect the retrieved values. |
2015
|
T Yang, G Nava, P Minzioni, M Veglione, F Bragheri, F D Lelii, R M Vazquez, R Osellame, I Cristiani Investigation of temperature effect on cell mechanics by optofluidic microchips Journal Article Biomedical Optics Express, 6 (8), pp. 2991–2996, 2015. Abstract | Links | Tags: LabOnChip, Microfluidic chip, Optofluidics @article{Yang2015a,
title = {Investigation of temperature effect on cell mechanics by optofluidic microchips},
author = {T Yang and G Nava and P Minzioni and M Veglione and F Bragheri and F D Lelii and R M Vazquez and R Osellame and I Cristiani},
doi = {10.1364/BOE.6.002991},
year = {2015},
date = {2015-01-01},
journal = {Biomedical Optics Express},
volume = {6},
number = {8},
pages = {2991--2996},
abstract = {Here we present the results of a study concerning the effect of temperature on cell mechanical properties. Two different optofluidic microchips with external temperature control are used to investigate the temperature-induced changes of highly metastatic human melanoma cells (A375MC2) in the range of ~0 – 35 °C. By means of an integrated optical stretcher, we observe that cells' optical deformability is strongly enhanced by increasing cell and buffer-fluid temperature. This finding is supported by the results obtained from a second device, which probes the cells' ability to be squeezed through a constriction. Measured data demonstrate a marked dependence of cell mechanical properties on temperature, thus highlighting the importance of including a proper temperature-control system in the experimental apparatus.},
keywords = {LabOnChip, Microfluidic chip, Optofluidics},
pubstate = {published},
tppubtype = {article}
}
Here we present the results of a study concerning the effect of temperature on cell mechanical properties. Two different optofluidic microchips with external temperature control are used to investigate the temperature-induced changes of highly metastatic human melanoma cells (A375MC2) in the range of ~0 – 35 °C. By means of an integrated optical stretcher, we observe that cells' optical deformability is strongly enhanced by increasing cell and buffer-fluid temperature. This finding is supported by the results obtained from a second device, which probes the cells' ability to be squeezed through a constriction. Measured data demonstrate a marked dependence of cell mechanical properties on temperature, thus highlighting the importance of including a proper temperature-control system in the experimental apparatus. |
