T Yang, P Paiè, G Nava, F Bragheri, R M Vazquez, P Minzioni, M Veglione, M Di Tano, C Mondello, R Osellame, R Osellame, I Cristiani An integrated optofluidic device for single-cell sorting driven by mechanical properties Journal Article Lab on a Chip, 15 (5), pp. 1262–1266, 2015. Abstract | Links | Tags: LabOnChip, Optofluidics @article{Yang2015,
title = {An integrated optofluidic device for single-cell sorting driven by mechanical properties},
author = {T Yang and P Pai{è} and G Nava and F Bragheri and R M Vazquez and P Minzioni and M Veglione and M {Di Tano} and C Mondello and R Osellame and R Osellame and I Cristiani},
doi = {10.1039/c4lc01496k},
year = {2015},
date = {2015-01-01},
journal = {Lab on a Chip},
volume = {15},
number = {5},
pages = {1262--1266},
abstract = {We present a novel optofluidic device for real-time sorting on the basis of cell mechanical properties, measured by optical stretching. The whole mechanism, based on optical forces, does not hamper the viability of the tested cells, which can be used for further analysis. The device effectiveness is demonstrated by extracting a sample population enriched with highly metastatic cells from a heterogeneous cell mixture.},
keywords = {LabOnChip, Optofluidics},
pubstate = {published},
tppubtype = {article}
}
We present a novel optofluidic device for real-time sorting on the basis of cell mechanical properties, measured by optical stretching. The whole mechanism, based on optical forces, does not hamper the viability of the tested cells, which can be used for further analysis. The device effectiveness is demonstrated by extracting a sample population enriched with highly metastatic cells from a heterogeneous cell mixture. |
T Yang, G Nava, P Minzioni, M Veglione, F Bragheri, F D Lelii, R M Vazquez, R Osellame, I Cristiani Investigation of temperature effect on cell mechanics by optofluidic microchips Journal Article Biomedical Optics Express, 6 (8), pp. 2991–2996, 2015. Abstract | Links | Tags: LabOnChip, Microfluidic chip, Optofluidics @article{Yang2015a,
title = {Investigation of temperature effect on cell mechanics by optofluidic microchips},
author = {T Yang and G Nava and P Minzioni and M Veglione and F Bragheri and F D Lelii and R M Vazquez and R Osellame and I Cristiani},
doi = {10.1364/BOE.6.002991},
year = {2015},
date = {2015-01-01},
journal = {Biomedical Optics Express},
volume = {6},
number = {8},
pages = {2991--2996},
abstract = {Here we present the results of a study concerning the effect of temperature on cell mechanical properties. Two different optofluidic microchips with external temperature control are used to investigate the temperature-induced changes of highly metastatic human melanoma cells (A375MC2) in the range of ~0 – 35 °C. By means of an integrated optical stretcher, we observe that cells' optical deformability is strongly enhanced by increasing cell and buffer-fluid temperature. This finding is supported by the results obtained from a second device, which probes the cells' ability to be squeezed through a constriction. Measured data demonstrate a marked dependence of cell mechanical properties on temperature, thus highlighting the importance of including a proper temperature-control system in the experimental apparatus.},
keywords = {LabOnChip, Microfluidic chip, Optofluidics},
pubstate = {published},
tppubtype = {article}
}
Here we present the results of a study concerning the effect of temperature on cell mechanical properties. Two different optofluidic microchips with external temperature control are used to investigate the temperature-induced changes of highly metastatic human melanoma cells (A375MC2) in the range of ~0 – 35 °C. By means of an integrated optical stretcher, we observe that cells' optical deformability is strongly enhanced by increasing cell and buffer-fluid temperature. This finding is supported by the results obtained from a second device, which probes the cells' ability to be squeezed through a constriction. Measured data demonstrate a marked dependence of cell mechanical properties on temperature, thus highlighting the importance of including a proper temperature-control system in the experimental apparatus. |
